A A Review on the Potential of Application of CRISPR/Cas9 Technology in CYP3A4 and CYP2D6 Genes: A Way to Move Forward in Identifying New Drug Targets

The cytochrome P450 (CYP) enzymes are of a ubiquitous heme–thiolate proteins family that plays an important role within the biotransformation endogenous molecules and xenobiotics as today’s market is bio-transformed through CYP-mediated metabolism. Major CYP isoforms involved in most reactions mainly CYP3A4 CYP2D6. found extensively human liver gut which this CYP-isoform takes part phase I transformation toxins, carcinogens, bile acids, steroid hormones while CYP2D6 central oxidative metabolism up to 25% drugs common clinical use, although it only accounts for 1-5% content. With help vast gene editing technologies such CRISPR/Cas9 system, researchers have its application be useful efficient knockout studies involving genetic variants association with drug due simplicity affordability. Hence, review aims explore potential use technology vitro particularly knocking out interest, enzymes. This article will how CRISPR/cas9 facilitates ex vivo assays studies. long-term goal bio-editing treatment without risk causing harm humans providing ready-to-be-used assay development pharmacokinetics study at lower cost.